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. 2008 Dec 23;80(4):771–780. doi: 10.1095/biolreprod.108.072843

FIG. 2.

FIG. 2.

Testicular hyperthermia results in serine phosphorylation of BCL2 in germ cells. I and II) Portions of stage XII tubules from control and a rat that had been exposed to short-term local testicular heating shows serine phosphorylation of BCL2 only in heat-susceptible late pachytene spermatocytes 6 h after heating (II). Bar = 25 μm. (IIIV) Confocal images of late pachytene spermatocytes at stage XII from a heat-treated rat show TUNEL (green), phospho-BCL2 (red), and colocalization of TUNEL and phospho-BCL2 (yellow) in apoptotic germ cells 6 h after heat treatment. Bar = 50 μm.