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. Author manuscript; available in PMC: 2010 Dec 27.
Published in final edited form as: Transplantation. 2009 Dec 27;88(12):1341–1348. doi: 10.1097/TP.0b013e3181bcde7b

Figure 3. Physiologic effects of adoptive transfer of col(V) specific effector and regulator cells.

Figure 3

(A) Swelling of the rat ear 24 hours after injection of col(V) into the ear and adoptive transfer of Te or Te+Tr cells. HEL was used as a control antigen. (B, C) Expression of IL-17 (B) and IL-23 (C) in mediastinal lymph nodes 7 and 14 days after transplantation and adoptive transfer of LNCs and SPCs. qPCR for the cytokines IL-17 (B) and IL-23 (C). (D) FoxP3 expression in BAL and mediastinal lymph node cells from animals that obtained effector cells (Te) or effector cells (black solid and black hatched bars) and regulatory cells (Te + Tr) (gray solid and grey hatched bars). ΔΔCT with an external standard was calculated relative to cyclophilin and the fold change (2−ΔΔCT) in mRNA steady state levels between the different groups was calculated. * and ** indicates a significant difference between the data denoted (p<0.05). All values are shown as the mean of 3 to 5 independent experiments ± standard deviation.