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. Author manuscript; available in PMC: 2010 Jan 12.
Published in final edited form as: Toxicol Appl Pharmacol. 2009 Jan 23;236(2):154–165. doi: 10.1016/j.taap.2008.12.028

Fig. 3.

Fig. 3

Effect of Cr(III) and Cr(VI) on the in vitro activity of synthesome-associated DNA polymerase α, δ and ε. Polymerase assays were performed in the absence or presence of a range of concentrations of chromium chloride (▲) or sodium dichromate (◆) ion, under the reaction conditions described in the Methods. (A) Polymerase α activity was reported as the percentage of 3H-dTTP incorporated into an activated calf thymus DNA template. (B) Polymerase δ activity was reported as the percentage of 3H-dTTP incorporated into a poly(dA-dT).poly(dA-dT) DNA template. (C) Polymerase ε activity was reported as the percentage of 3H-dTTP incorporated into a poly(dA).oligo(dT) DNA template. All values reported in the figure are the average of three independent experiments, and are reported as a percentage of the radiolabeled nucleotide incorporated into the nascent DNA during the polymerase reaction, relative to a control reaction which was performed in the absence of chromium ion.