Table 4.
Summary of the frequency and type of mutations produced by the DNA synthesome during the in vitro DNA replication reactions performed in the presence of chromium ion
| Mutation type | Cr(III)-treated number (%) | Cr(VI)-treated number (%) | Spontaneous number (%) | All |
|---|---|---|---|---|
| Substitutionsb | 23(63.4%) | 14(63.6%) | 12(70.6%) | 49(65.3%) |
| Transitiona | 7(19.4) | 6(27.3) | 7(41.2) | 20 |
| GcàAT | 4(11.1) | 4(18.2) | 7(41.2) | 15 |
| AtàGC | 3(8.3) | 2(9.1) | 0 | 5 |
| Transversiona | 16(44.4) | 8(36.4) | 5(29.4) | 29 |
| GcàTA | 7(19.4) | 2(9.1) | 2(11.8) | 11 |
| GCàCG | 5(13.8) | 3(13.6) | 2(11.8) | 10 |
| AtàCG | 3(8.3) | 2(9.1) | 1(5.9) | 6 |
| AtàTA | 1(2.8) | 1(4.5) | 0 | 2 |
| Deletionsb | 3(8.3%) | 2(9.1%) | 1(5.9%) | 6(8.0%) |
| Insertionsb | 10(27.8%) | 6(27.3%) | 4(23.5%) | 20(26.7%) |
| All Mutations | 36(100%) | 22(100%) | 17(100%) | 75(100%) |
The types and frequency of mutations induced by Cr(III) and Cr(VI) ion, during the in vitro DNA replication reaction was determined as described in the Methods. The data reported were obtained from three independent experiments.
indicates that there is a significant difference between the frequency of a specific type of mutation arising from synthesome mediated DNA replication reactions containing either chromium chloride, sodium dichromate, or lacking chromium ion; (p<0.005)
indicates that there is no significant difference between the frequency of specific types of mutations arising from synthesome mediated DNA replication reactions either containing or lacking these metal ions; (p>0.05).