Skip to main content
. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: J Proteome Res. 2009 Nov;8(11):5031–5040. doi: 10.1021/pr900452s

Figure 7.

Figure 7

Comparison of concentration of carbohydrate probes required for effective immobilization on hydrazide-dendrimer coated slide under 10 mins microwave treatment and normal water-bath heating. ~1 nL of Sialyl Lex from concentration of 100 no to 100 µM in Formamide was arrayed on the slides and subjected to (A) 10 mins microwave treatment, and (B) by water-bath heating (50 °C, 16 hrs). The treated slides were incubated with 35 µL of 1 Ug/mL mixture of Biotin-labeled anti-Sialyl Lex antibody and Cy5-Streptavidin solution at 30 °C for 60 min. The microarrays were washed with PBST buffer for 5 mins twice and 0.01 × PBS for 1 min. Images were scanned with confocal scanner under same laser power and PMT, and were recorded in rainbow color display for better vision with naked eyes.