Figure 9.

Specificity of carbohydrate-lectin/antibody interactions on the carbohydrate microarrays fabricated on the hydrazide-dendrimer coated slide by microwave assisted immobilization. Spotted carbohydrate microarrays were blocked with 0.1× PBS buffer containing 1% BSA, 0.1% Tween 20 after treatment for 10 mins microwave radiation. The microarrays were then incubated with either a cocktail of 1 µg/mL of biotin-labeled lectins of ConA, AA. WGA and anti-Sialyl Le^x antibody or individual lectins/antibody solution at 30 °C for 60 min, respectively, followed by stained with 0.1 µg/mL of Cy3-streptavidin at 30 °C for 60 min. The microarrays were washed with PBST buffer for 5 mins twice and 0.01 × PBS for 1 min before they scanned with confocal scanner under identical layer power and PMT. The binding profiles showed that all these interactions have been detected successfully, indicating the retention of specific binding activity of the immobilized carbohydrates.