Figure 4.

Effects of varying ligand induction on autocrine cell ECAR. Autocrine cells were plated onto Cytosensor transwells and were either fully induced with no tetracycline (—▴—) or partially induced with 5 ng/ml of tetracycline (—•—). Noninduced cells (1 ng/ml tetracycline) were used as controls (—■—, —♦—). Steady–state ECAR was set to 100%, and the cells received the indicated concentration of anti-EGFR antibody except for control cells receiving no additions (—■—).