Table 1.
Primary antibodies used for immunostaining
| Anitgen | Immunogen | Manufacturing details | Working dilution |
|---|---|---|---|
| CCK | Synthetic human gastrin/CCK 2–17 conjugated with carbodiimide to keyhole limpet hemocyanin | UCLA Cure (Los Angeles, CA), mouse monoclonal, 9303 | 1:1000 |
| CR | Human recombinant calretinin | Swant (Bellinzona, Switzerland), goat polyclonal, CG1 | 1:500 |
| CR | Human recombinant calretinin | Swant, rabbit polyclonal, 7699/4 | 1:1000 |
| GABA | (γ-Aminobutyric Acid)-BSA | Sigma-Aldrich (St. Louis, MO), rabbit polyclonal, A2052 | 1:5000 |
| GFP | Purified recombinant green fluorescent protein | Aves Labs (Tigard, OR), chicken polyclonal, GFP-1020 | 1:500 |
| NOS | Synthetic peptide corresponding to amino acid residues 1414–1429 of the rat NOS-I protein | Millipore/Chemicon (Temecula, CA), rabbit polyclonal, AB1552 | 1:250 |
| NPY | Synthetic porcine neuropeptide tyrosine (NPY) | Millipore/Chemicon, rabbit polyclonal, AB1915 | 1:1000 |
| NPY | Synthetic NPY | Millipore/Chemicon, sheep polyclonal, AB1583 | 1:1000 |
| PV | Rat muscle parvalbumin | Swant, goat polyclonal, PVG-214 | 1:1000 |
| PV | Rat muscle parvalbumin | Swant, rabbit polyclonal, PV-28 | 1:1000 |
| SOM | Synthetic 1–14 cyclic somatostatin | Millipore/Chemicon, rat monoclonal, MAB354 | 1:200 |
| VIP | Porcine VIP conjugated to bovine thyroglobulin with carbodiimide | ImmunoStar, Inc. (Hudson, WI), rabbit polyclonal, 20077 | 1:500 |