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. Author manuscript; available in PMC: 2010 Dec 8.
Published in final edited form as: Cancer Cell. 2009 Dec 8;16(6):463–474. doi: 10.1016/j.ccr.2009.10.016

Figure 2. p85α mutants interact with p110α and stabilize p110α.

Figure 2

(A-D) HA-tagged p85α mutants and wild-type p85α that carry an intact p110α interaction domain immunoprecipitate p110α from Cos7 cells transfected with the constructs indicated in the panels. Expression of all the transfected constructs was confirmed in the lysates as shown below in each of the panels. The dominant negative p85Δ and empty vector transfected cells served as controls. (E) p85α mutants that contain an intact p110α binding domain when transiently expressed in pan-p85 null MEFs stabilize p110α levels.