FIG. 3.

p53 Mutant zebrafish are sensitive to UVB irradiation. (A) Comparison of sensitivity of 6-day-old wt and p53 mutant fish at various UVB doses. Ten fish were used at each dose in each experiment. Experiments had three replicates. Means and standard deviations of the three experiments are presented. (B) Comparison of sensitivity in 5–6-week-old wt and p53 fish at various UVB doses. (C) The data from 6-day-old wt and p53 fish fit to linear regression lines (R² = 0.977 and 0.9352 for wt and p53 fish, respectively; R² = 1 when curves best fit the regression line). The calculated LD50s (where survival is 50%) are 0.6 kJ/m² for both wt and p53 fish. (D) The data from 5–6-week-old wt and p53 young adults fit to polynomial regression lines (R² = 0.9512 and 0.9747 for wt and p53 fish, respectively). (E, F) The typical morphology of UVB-exposed zebrafish. Curved spinal is the common abnormalities in both juvenile and young adult fish, also in wt and p53 mutant fish.