Table 1.
Polymerase Chain Reaction Primers and Conditions
| |
|
|
PCR conditions |
|
|
|---|---|---|---|---|---|
| Gene | Mechanism | NCBI accession number | Annealing temperature (°C) | Cycles | Primers (5′–3′) |
| β-Actin | Cytoskeleton | AF057040 | 58 | 28 | F: TGCCATGTATGTGGCCATCCA |
| R: ACCTCCAGACAGCACTGTGT | |||||
| Caspase3a | Apoptosis | NM131877 | 57 | 31 | F: TGTGTTGCTCAGTCACGGC |
| R: GGCATGTTGGAGGTGGACTC | |||||
| DDB2 | DNA repair (NER) | NM001083061 | 57 | 38 | F: AAAAGACCGAATGAAGAAACTCC |
| R: TAGTAAGCAACTTGGTGCTGTCA | |||||
| Ku80 | DNA repair (NHEJ) | AY877316 | 57 | 31 | F: TGGAGGAGATTGAGAGAGAACTG |
| R: GTTTCATCATCATCGTTCAGACA | |||||
PCR, polymerase chain reaction; DDB, damaged DNA binding proteins; NER, nucleotide excision repair; NHEJ, nonhomologous end joining; NCBI, National Center for Biotechnology Information.