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. Author manuscript; available in PMC: 2010 Jan 12.
Published in final edited form as: Nature. 2008 Aug 24;455(7213):674–678. doi: 10.1038/nature07317

Fig 1. STING is an ER protein.

Fig 1

a. Schematic of hSTING indicating transmembrane and leucine rich regions. b. Immunoblot analysis of STING in HEK 293 cells treated with RNAi to STING (hSTING) or control RNAi (NS). c. Northern blot analysis of human STING. d. Confocal analysis of HEK 293 cells transfected with hSTING tagged at the carboxyl end with HA. Transfected cells were also analyzed using ER-dsRed, Mitotracker or Golgi-dsRed. e. Fractionation experiments confirm that STING resides in the ER. Control antibodies indicate accuracy of fractionation (Calreticulin- ER, COX IV-mitochondria, β-actin- cytosol).