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. Author manuscript; available in PMC: 2010 Jan 12.
Published in final edited form as: J Immunol. 2006 Nov 15;177(10):7164–7172. doi: 10.4049/jimmunol.177.10.7164

FIGURE 1.

FIGURE 1

Effect of TLR ligands on the up-regulation of BAFF and APRIL in airway epithelial cells. BEAS-2B cells (A) or PBEC (B) were incubated for 18 h with 10 μg/ml PGN, 100 μg/ml zymosan, 25 μg/ml dsRNA, 1 μg/ml LPS, 10 ng/ml flagellin, 1 μg/ml R-848, or 3 μg/ml CpG-A, as indicated, and then mRNA was extracted and analyzed for BAFF and APRIL using real-time PCR. C, BEAS-2B cells or PBEC were incubated for 18 h with 25 μg/ml dsRNA, and then expression of mRNA for full-length BAFF (246 bp), ΔBAFF (189 bp), and GAPDH (226 bp) was analyzed by conventional RT-PCR. D, BEAS-2B cells were incubated for 6 h with 0.25–25 μg/ml dsRNA, and then expression of mRNA for BAFF was analyzed by real-time PCR. E, BEAS-2B cells were incubated with 25 μg/ml dsRNA (circle) or vehicle control (square) for 0.5–18 h, and then expression of mRNA for BAFF was analyzed by real-time PCR. The copy number is expressed as the number of transcripts/ng total RNA. The results are shown as the mean ± SEM of three to five independent experiments. *, p < 0.05.