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. Author manuscript; available in PMC: 2010 Jan 12.
Published in final edited form as: J Immunol. 2006 Nov 15;177(10):7164–7172. doi: 10.4049/jimmunol.177.10.7164

FIGURE 7.

FIGURE 7

Effect of epithelial cell-derived BAFF on the cell survival of B cells. A, Purified human B cells were stimulated with 5 ng/ml BAFF or concentrated BEAS-2B supernatant for 5 days. B, Purified human B cells were stimulated with 5 ng/ml BAFF, 10 ng/ml IL-4, 10 μg/ml mouse anti-human CD40 mAb, concentrated supernatant, or the indicated combinations for 5 days in the presence or absence of 5 μg/ml control IgG1, 5 μg/ml BAFF-R:Fc, or 5 μg/ml TACI:Fc. MTT reagent was added for the last 4 h of incubation. The concentrated BEAS-2B supernatant was added at a final dilution of 1/20 to the B cell culture. Absorbance at 570 nm was measured using a microtiter plate reader. The results are shown as the mean ± SEM of five independent experiments.*, p < 0.05.