Table 2.
Oligosaccharides in the fraction eluted from the solid phase with a 90:10 deionized water-acetonitrile solution and their constituent monosaccharidesa. Data were obtained using a nESI-FTICR instrument in negative ion-detection mode and tandem mass spectrometry. Oligosaccharide compositions were assigned using an in-home Glycan Finder program.
| m/z [M-H]− |
Hex | HexNAc | NeuAc | NeuGc | relative intensity |
|---|---|---|---|---|---|
| 632.10 | 2 | 1 | 100 | ||
| 794.03 | 3 | 1 | 13.72 | ||
| 964.90 | 1 | 1 | 2 | 0.72 | |
| 1158.90 | 4 | 1 | 1 | 0.3 | |
| 706.02 | 3 | 1 | 0.3 | ||
| 809.96 | 3 | 1 | 0.24 | ||
| 867.99 | 4 | 1 | 0.18 | ||
| 1038.85 | 2 | 2 | 1 | 0.18 |
a
Hex, hexose; HexNAc, N-acetylhexosamine; NeuAc, N-acetyl-neuraminic acid (sialic acid); NeuGc, N-glycolylneuraminic acid.