Table 4.
Oligosaccharides in the fraction eluted from the solid phase with a 60:40 deionized water-acetonitrile solution containing 0.1% trifluoroacetic acid and their constituent monosaccharidesa. Data obtained using an nESI-FTICR instrument in negative ion-detection mode and tandem mass spectrometry. Oligosaccharide compositions were assigned using an in-home Glycan Finder program.
| M/z [M-H]− |
Hex | HexNAc | NeuAc | Relative intensity |
|---|---|---|---|---|
| 632.12 | 2 | 1 | 83.89 | |
| 794.12 | 3 | 1 | 5.83 | |
| 834.54 | 2 | 1 | 1 | 5.22 |
| 673.16 | 1 | 1 | 1 | 4.75 |
a
Hex, hexose; HexNAc, N-acetylhexosamine; NeuAc, N-acetyl-neuraminic acid (sialic acid).