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. Author manuscript; available in PMC: 2010 Jan 12.
Published in final edited form as: Scand J Immunol. 2008 Jul;68(1):30–42. doi: 10.1111/j.1365-3083.2008.02113.x

Figure 6.

Figure 6

Accumulation of fatty acids into triglyceride-rich lipid droplets protects against changes in antigen presentation. (A) Confocal fluorescence microscopy images of C1C12 BODIPY (green), Nile Red (red), or C16BODIPY (yellow) in T2-Kb cells treated for 12 h with BSA, 500 μM PA, 500 μM OA, or 500 μM PA + 500 μM OA. Images are false coloured and the nucleus is stained with DAPI (blue). C1C12BODIPY and Nile Red report on accumulation of monounsaturated fatty acids in neutral lipid stores and C16BODIPY reports on incorporation of palmitate. (B) Box plot statistics for the fluorscence microscopy data represented in A for n = 31–43 cells per treatment condition. The frequency is area (pixels2) occupied by C1C12BODIPY, Nile red, or C16BODIPY in lipid droplets of T2-Kb cells above a defined threshold. Note that non-overlapping notches (dashed line) represent statistical significance [27].