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Fluorescence micrographs of microchip channels during a 1.0 s gated injection. Reservoir 1 was filled with high concentration (mM) fluorescein solution, and the other reservoirs were filled with buffer. The loading volume during the 1.0 s injection was estimated to be ~750 pL, by calculating the volume of a rectangular block 930 μm long, 62 μm wide, and 13 μm high. The scale bar in the 0.0 s frame applies to all images.
