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. Author manuscript; available in PMC: 2010 Jan 12.
Published in final edited form as: ACS Chem Biol. 2008 Jul 18;3(7):437–448. doi: 10.1021/cb800057j

Figure 2.

Figure 2

a) MazEF associates with its promoter sequence bound to the PC biosensor surface in a dose-dependent fashion. b) Preincubation of MazEF (1.8 μM) with its nonbiotinylated promoter sequence reduces the association of MazEF with the promoter-bound biosensor surface. c) Kinetics of MazEF (0.2 mg mL-1) binding to its own promoter sequence. A rapid increase in PWV shift is observed upon MazEF addition to the promoter-bound biosensor surface. In contrast, MazEF showed little affinity for a biotinylated alternating GC control DNA of the same length as its promoter sequence, similar to its association blocked sensor surface (no DNA). All error bars represent the calculated standard error (n = 3).