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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cancer Res. 2009 Dec 8;70(1):378–387. doi: 10.1158/0008-5472.CAN-09-2021

Fig. 1. Effect of miR-145 on cell invasion and lung metastasis.

Fig. 1

A & B, Stably transduced MDA-MB-231 or LM2-4142 cells ectopically expressing miR-145 (145) or vector alone (V) were subject to matrigel chamber assay as detailed in Materials and Methods. Invaded cells on the membrane were counted as follows. Since the distribution of cells on the membrane was not always even, we first took a picture at a low magnification and then enlarged the image in a computer screen with grids so that all of the cells on the entire membrane were counted. B & D, Lung metastasis as revealed by the experimental metastasis animal model. Stably transduced MDA-MB-231 (B) or LM2-4142 (D) cells ectopically expressing miR-145 (145) or vector alone (V) were introduced into female nude mice through tail vein as described in Materials and Methods. Values A and C are means ± SE of three independent experiments. **, p < 0.01; SC, scrambled oliog; anti-145, anti-miR-145.