Skip to main content
. Author manuscript; available in PMC: 2010 Jan 12.
Published in final edited form as: Development. 2007 Jan 3;134(3):515–523. doi: 10.1242/dev.02763

Fig. 7. Nkx2.2 interacts with Grg3 in the embryonic pancreas.

Fig. 7

(A-D) RNA in situ hybridization for Grg3 at E12.5 (A) and E16.5 (C,E,F), and for Nkx2.2 at E12.5 (B) and E16.5 (D). Adjacent sections demonstrate similar expression patterns throughout pancreas development. In C and D, outlines represent selected overlapping expression areas. (E,F) Combined RNA in situ hybridization and immunohistochemistry on E16.5 pancreata demonstrate overlapping expressions between Grg3 (blue) and glucagon (brown; arrows in E), and distinct expression domains between Grg3 (blue) and amylase (brown; F). (G) Co-immunoprecipitation for Nkx2.2 and Grg3: PANC cell lysates were incubated with Grg3 antibody and were western blotted with antibodies against Nkx2.2. Lanes 1 and 4: vector-only control; lanes 2 and 5: Nkx2.2 transfected with Grg3; lanes 3 and 6: Nkx2.2ΔTN transfected with Grg3. Lanes 1-3 are input samples and lanes 4-6 are Grg3 pull-down samples. Nkx2.2 is co-immunoprecipitated with Grg3 (lane 5).