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. Author manuscript; available in PMC: 2010 Jan 23.
Published in final edited form as: Cell. 2009 Jan 23;136(2):284–295. doi: 10.1016/j.cell.2008.11.037

Figure 2. Expression of scaffold nucleoporins is down-regulated during C. elegans development.

Figure 2

(A). Scheme of C. elegans strains used in this work (based on (Crittenden et al., 2006)). N2 is wild type strain, CF512 (fem-1, fer-15) has thermo-sensitive mutations that inhibit male germline production at 25°C, SS104 (glp-4) has a mutation that inhibits male and female germline production at 25°C, being adults worms at the restrictive temperature entirely post-mitotic. (B) Total RNA was extracted from the embryo, larva and adult developmental stages of C. elegans (SS104 strain). Expression levels were analyzed by RT-PCR (n=4) and normalized to embryonic levels. Cyclin B (CycB) was used as a marker for cell cycle exit. Standard deviation is below 20%. (C) Nucleoporin expression levels in embryos (E), larvae (L) and adults (A) worms of C. elegans sterile strains CF5120 and SS104 were analyzed by RT-PCR (n=4).

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