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. Author manuscript; available in PMC: 2010 Jan 23.
Published in final edited form as: Cell. 2009 Jan 23;136(2):284–295. doi: 10.1016/j.cell.2008.11.037

Figure 3. Expression of scaffold nucleoporins is not required during adulthood.

Figure 3

(A). daf-2(e1370) mutant worms on day 1 of adulthood were fed bacteria expressing empty vector control (gray line), ceNup153 RNAi (red line), ceNup160 RNAi (light blue line) ceNup107 RNAi (blue line) or ceLamin RNAi (green line). Life span was assessed as described (Apfeld and Kenyon, 1998). Statistical analyses can be found in Table S1. (B) Total protein was extracted from sterile (25°C) adults SS104 worms subject to the indicated RNAis for 3 or 6 days and protein levels were assayed by western blot analysis. Values represent average ± SD from 3 independent experiments. (C) Total protein was extracted from a reproductive population (15°C) of SS104 worms subjecteded to the indicated RNAis for 3 days and protein levels were assayed by western blot. Values represent average ± SD from 3 independent experiments.

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