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. Author manuscript; available in PMC: 2010 Jan 12.
Published in final edited form as: Eur J Neurosci. 2009 Jan;29(2):287–306. doi: 10.1111/j.1460-9568.2008.06590.x

Fig. 3. Rap1 and B-Raf are activated in DA and SKF R-38393-treated strital neurons via D1 receptor and PKA pathway.

Fig. 3

Neurons were treated for 10 min with 10 μM and 50 μM of DA or SKF R-38393. Rap1 activation [A] and B-Raf kinase activity [B] were conducted as described in Experimental Procedures. C. Striatal neurons were pretreated for 15 min with vehicle or with the following compounds: SCH 23390 (1 μM), SMBS (20 μM), KT5720 (1 μM), then treated with 10 μM of SKF R-38393 for 10 min. B-Raf kinase activity analyses were conducted. In bar graph, blots were scanned and values expressed as the average ± S.E.M of three experiments (n=3). p<0.05 (#), represents the result of paired Student’s t test with 4 degrees of freedom for the corresponding control group.