Fig. 4. D2-like DA receptors, β-adrenergic receptors and NMDA receptors are not required in DA and SKF R-38393-induced ERK activation in striatal neurons.
Striatal neurons were pretreated for 15 min with vehicle or with the following compounds: spiperone (10 μM), propranolol (10 μM) or MK801 (10 μM), and then treated with 10 μM of either DA [A] or SKF R-38393 [B] for 20 min. Immunoblot analysis of p-ERK1/2 expression was conducted; data shown are the average ± SEM of three independent experiments (n=3). In bar graph, P<0.05 (*), represents the result of paired Student’s t test with 4 degrees of freedom for the corresponding agonist (DA or SKF R-38393) alone-treated group; p<0.05 (#), represents the result of paired Student’s t test with 4 degrees of freedom for the corresponding control group.