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. 1998 Dec 22;95(26):15388–15393. doi: 10.1073/pnas.95.26.15388

Figure 1.

Figure 1

Suppression of gsp1 by MOG1 and PDE2. (A and B) Restriction enzyme sites and ORFs of the yeast genomic DNA insert carried on pGMS28–479 (A) and pGMS4–479 (B) are shown. The ORFs carried on these plasmids were subcloned into the indicated plasmids by using the appropriate enzyme sites of YEplac195 and introduced into the N43–6C-gsp1–479 strain. Ura+ transformants were streaked on a synthetic-medium (ura−) plate and incubated at either 26°C or 34°C for 3 days as indicated. +, full suppression. −, no suppression. The restriction enzyme sites shown are as follows. B, BamHI; H, HindIII; Pv, PvuII; P, PstI; Sc, ScaI; Sp, SpeI; S, SacI; BII, BglII; K, KpnI; Sh, SphI; E105, Eco105I; E, EcoRI. (c) Amino acid sequence of Mog1p predicted from the sequence of pYJR074W.