Table 1.
Yeast strains and plasmids used in this study
| Relevant markers | Genotype/comment | Source | |
|---|---|---|---|
| Yeast | |||
| N43-6C-gsp1-479 | MATa Δgsp1∷HIS3∷gsp1ts∷LEU2 ade2 leu2 trp1 ura3 | (11) | |
| PSY962 | MATα Δgsp1∷HIS3 Δgsp2∷HIS3 ura3-52 leu2-Δ1 his3- Δ200 trp1-Δ63 covered with GSP1 on a ura3-based vector | (10) | |
| PSY962-479 | MATα Δgsp1∷HIS3 Δgsp2∷HIS3 ura3-52 leu2-Δ1 his3-Δ 200 trp1-Δ63 [pF314gsp1-479] | This study | |
| PSY852 | MATa Δntf2∷HIS3 ura3-52 leu2-Δ1 covered with NTF2 on a ura3-based vector | (27) | |
| NN19-5B | MATa rna1-1 ade2 leu2 his3 trp1 ura3 | (34) | |
| YPH499 | MATa ade2-101 leu2-Δ 1 trp1-Δ63 ura3-52 lys2-801 his3-Δ200 | (45) | |
| YPH500 | MATα ade2-101 leu2-Δ1 trp1-Δ63 ura3-52 lys2-801 his3-Δ200 | (45) | |
| MOY1 | MATa Δmog1∷HIS3 ade2-101 leu2-Δ 1 trp1-Δ63 ura3-52 lys2-801 his3-Δ200 | This study | |
| MOY2 | MATα Δmog1∷HIS3 ade2-101 leu2-Δ1 trp1-Δ63 ura3-52 lys2-801 his3-Δ200 | This study | |
| Yeast plasmids | |||
| p195GSP1 | 2 μm URA3 GSP1 | YEplac195 with 1.95-kb GSP1 fragment at KpnI/PstI site | This study |
| p195NTF2 | 2 μm URA3 NTF2 | YEplac195 with 1 kb NTF2 fragment at XbaI/EcoRI site | This study |
| p195MOG1 | 2 μm URA3 MOG1 | YEplac 195 with 1.3-kb MOG1 fragment at SmaI/PstI site | This study |
| p195PDE2 | 2 μm URA3 PDE2 | YEplac195 with 4.2-kb PDE2 fragment at KpnI/BamHI site | This study |
| pRSCNR1 | CEN URA3 CNR1/GSP1 | pRS316 with CNRI/GSP1 fragment at BamHI/SalI site | (17) |
| p316NTF2 | CEN URA3 NTF2 | pRS316 with 1-kb NTF2 fragment at XbaI/EcoRI site | This study |
| p316MOG1 | CEN URA3 MOG1 | pRS316 with 1.1-kb MOG1 fragment at SacI/SmaI site | This study |
| p316PDE2 | CEN URA3 PDE2 | pRS316 with 3-kb PDE2 fragment at BamHI/HindIII site | This study |
| pFB1-33C | 2 μm URA3 | H2B NLS fused in-frame to β-galactosidase | (38) |
| GAL10∷H2B∷lacZ | |||
| pPS919 | CEN LEU2 ntf2-1 | pRS315 with ntf2-1 | (27) |
| pPS920 | CEN LEU2 ntf2-2 | pRS315 with ntf2-2 | (27) |
| pPS751 | 2 μm URA3 Npl3-Myc | YEp352 with Myc-tagged Npl3p | (39) |
| E. coli plasmids | |||
| pGEX-GSP1 | GST-fused GSP1 for E. coli expression | (34) | |
| pGEX-YRB1 Δ 1-9 | GST-fused YRB1 lacking N-terminal 9 amino acid residues for E. coli expression | (34) | |
| pGEX-MOG1 | GST-fused MOG1 for E. coli expression | This study | |
| pGEX-PRP20 | GST-fused PRP20 for E. coli expression | (34) | |
| pHIS3MOG1 | Disruption of MOG1 by replacement of 0.65-kb full-size MOG1 ORF with 1.75-kb HIS3 fragment | This study |