TABLE 1.
Efficiency of bacteriophage immobilization on magnetic and cellulose beadsa
| Type of support | No. of BCCP-T4 or CBM-T4 particles (log PFU/ml) |
% Particles bound | No. of T4 particles (log PFU/ml) |
% Particles bound | ||
|---|---|---|---|---|---|---|
| Initial | Supernatant | Initial | Supernatant | |||
| Magnetic beads (no.) | ||||||
| 2 × 109 | 9.09 ± 0.02 | 8.67 ± 0.07 | 62 ± 6 | 9.00 ± 0.05 | 8.98 ± 0.02 | 5 ± 6 |
| 7.31 ± 0.02 | 6.62 ± 0.06 | 79 ± 3 | ||||
| 1 × 109 | 9.30 ± 0.00 | 8.50 ± 0.11 | 84 ± 4 | 8.99 ± 0.02 | 8.98 ± 0.04 | 3 ± 8 |
| 7.30 ± 0.00 | 6.75 ± 0.05 | 72 ± 3 | ||||
| 5.30 ± 0.00 | 4.90 ± 0.03 | 60 ± 2 | ||||
| 2 × 108 | 9.21 ± 0.02 | 8.91 ± 0.07 | 49 ± 8 | |||
| 8.21 ± 0.02 | 7.93 ± 0.05 | 50 ± 5 | 8.07 ± 0.01 | 8.01 ± 0.01 | 13 ± 3 | |
| 7.21 ± 0.02 | 7.00 ± 0.01 | 37 ± 2 | 7.07 ± 0.01 | 7.01 ± 0.02 | 16 ± 4 | |
| Cellulose beads (mg) | ||||||
| 100 | 4.25 ± 0.04 | 2.55 ± 0.08 | 98 ± 0 | 7.01 ± 0.09 | 5.84 ± 0.33 | 92 ± 5 |
| 10 | 4.52 ± 0.16 | 4.15 ± 0.10 | 69 ± 9 | 4.45 ± 0.08 | 4.43 ± 0.04 | 4 ± 10 |
| 4.24 ± 0.16 | 3.61 ± 0.14 | 76 ± 8 | 4.15 ± 0.08 | 4.16 ± 0.04 | −3 ± 10 | |
| 3.94 ± 0.16 | 3.39 ± 0.08 | 71 ± 6 | 3.85 ± 0.08 | 3.91 ± 0.06 | −15 ± 16 | |
| 3.64 ± 0.16 | 3.17 ± 0.10 | 65 ± 8 | 3.55 ± 0.08 | 3.66 ± 0.05 | −30 ± 15 | |
a
Data (means ± standard deviations for 2 to 5 experiments) represent numbers of phage particles in the initial sample and in the supernatant (after bead removal). Results shown for magnetic beads were determined using BCCP-T4 phage, and results shown for cellulose beads were determined using CBM-T4 phage.