TABLE 2.
Sec translocon and Sec-dependent secreted proteins involved in biofilm formation
| Designation | Well IDa | Gene | Gene product | Biological process(es) | Sec secretionb |
|---|---|---|---|---|---|
| FTN_0090 | 4F07 | acpA | Acid phosphatase | Fatty acid and lipid metabolism | Secreted |
| FTN_0100 | 20C12 | Hypothetical membrane protein | Hypothetical, novel | Secreted | |
| FTN_0109 | 14G06 | Protein of unknown function | Unknown function, novel | Secreted | |
| FTN_0121 | 26G09 | secB1 | Preprotein translocase, subunit B | Motility, attachment and secretion, structure | Translocon |
| FTN_0121 | 4F06 | secB1 | Preprotein translocase, subunit B | Motility, attachment and secretion, structure | Translocon |
| FTN_0191 | 19E06 | Polar amino acid uptake transporter | Transport of amino acids | Secreted | |
| FTN_0304 | 20C11 | Pilus assembly protein | Motility, attachment and secretion, structure | Secreted | |
| FTN_0308 | 19H06 | Membrane protein of unknown function | Unknown function, novel | Secreted | |
| FTN_0357 | 21B08 | pal | Peptidoglycan-associated lipoprotein, OmpA family | Transport of drugs, antibacterial compounds | Secreted |
| FTN_0429 | 14G12 | Conserved protein of unknown function | Unknown function, conserved | Secreted | |
| FTN_0635 | 25C04 | Serine-type d-Ala-d-Ala carboxypeptidase | Cell wall, LPS, capsule | Secreted | |
| FTN_0672 | 12G03 | secA | Preprotein translocase, subunit A (ATPase, RNA helicase) | Motility, attachment and secretion, structure | Translocon |
| FTN_0713 | 14C04 | ostA2 | Organic solvent tolerance protein OstA | Cell wall, LPS, capsule | Secreted |
| FTN_0713 | 21H10 | ostA2 | Organic solvent tolerance protein OstA | Cell wall, LPS, capsule | Secreted |
| FTN_0713 | 26E07 | ostA2 | Organic solvent tolerance protein OstA | Cell wall, LPS, capsule | Secreted |
| FTN_0714 | 12G01 | Protein of unknown function | Unknown function, novel | Secreted | |
| FTN_0714 | 27C09 | Protein of unknown function | Unknown function, novel | Secreted | |
| FTN_1093 | 18A05 | Protein of unknown function | Unknown function, novel | Secreted | |
| FTN_1476 | 26A03 | Protein of unknown function | Unknown function, novel | Secreted | |
| FTN_1503 | 26A08 | Protein of unknown function | Unknown function, novel | Secreted | |
| FTN_1510 | 1E01 | secB2 | Preprotein translocase, subunit B | Motility, attachment and secretion, structure | Translocon |
| FTN_1630 | 13C11 | secG | Preprotein translocase, subunit G, membrane protein | Motility, attachment and secretion, structure | Translocon |
| FTN_1750 | 19H02 | Acyltransferase | Fatty acid and lipid metabolism | Secreted | |
| FTN_1750 | 23D04 | Acyltransferase | Fatty acid and lipid metabolism | Secreted |
a
Well identity (ID) annotation from the BEI Resources F. novicida two-allele transposon library.
b
Genes labeled translocon are structural components of Sec-dependent secretion. Secreted proteins were predicted using the SignalP algorithm.