TABLE 1.
Characteristics and locations of primers targeting Aeromonas enterotoxin genes
| Forward and reverse primersa | Locus | GenBank accession no. | Primer site location (5′-3′) |
Amplicon size (bp) | Reference or source | |
|---|---|---|---|---|---|---|
| Forward | Reverse | |||||
| AHCF (22) and AHCR (22) | act | M84709 | 1661-1682 | 1871-1892 | 232 | 5 |
| AHLF (22) and AHLR (22) | alt | L77573 | 686-707 | 1025-1046 | 361 | This study |
| AF092033 | 1782-1803 | 2121-2142 | 361 | |||
| U14011 | 1814-1835 | 2167-2147 | 361 | |||
| U63543 | 1988-2010 | 2341-2320 | 361 | |||
| CP000462 | 117050-117072 | 117389-117411 | 361 | |||
| DQ302128 | 581-602 | 920-941 | 361 | |||
| DQ302130 | 581-602 | 917-938 | 361 | |||
| DQ302131 | 581-602 | 917-938 | 361 | |||
| DQ302132 | 581-602 | 920-941 | 361 | |||
| DQ074979 | 581-602 | 920-941 | 361 | |||
| S65123 | 2128-2149 | 2460-2481 | 361 | |||
| AHSF (22) and AHSR (22) | ast | AF419157 | 2579-2600 | 3093-3114 | 536 | This study |
| CP000462 | 863088-863067 | 862553-862574 | 536 | |||
a
The sizes (in bp) of primers are given in parentheses.