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TEM of poly(3HP)-accumulating cells of E. coli HMS174(DE3)/pCOLADuet-1::dhaB1B2::pduP::phaC1. The fermentation was divided into an aerobic growth phase and an anaerobic production phase and was carried out using Riesenberg medium (19). The feeding solution for the anaerobic production phase was supplemented with 0.5 M di-Na+-fumarate, 0.5 M K+-Na+-tartrate, and 1 mM IPTG. Bars, 100 nm.
