FIG. 2.
Northern blot analysis to determine the expression of fur and lmo2185 from L. monocytogenes in response to 2,2′-dipyridyl. (a) Northern blot analysis for fur in the perR mutant in response to iron limitation by DPD. Ten micrograms of total RNA was isolated from parent cells grown in BHI medium (lane 1) or 0.3 mM DPD (lane 2) or the perR mutant grown in BHI medium (lane 3) or 0.3 mM DPD (lane 4). (b) Northern blot analysis of lmo2185 expression. The amounts of RNA and lanes are similar to those described above (a).