TABLE 1.
Strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Description or primer sequence (5′→3′)a | Source or reference |
|---|---|---|
| Strains | ||
| E. coli JM109 | recA1 supE44 hsdR17 gyrA96 relA1 thi (lac-proAB) F′ (traD36 proAB+lacIqZ M15) | This study |
| L. monocytogenes | ||
| 10403S | Serotype 1/2a | This study |
| Pfl | 10403S Pfur::lacZ fur+ | This study |
| Pf-1 | PFL fur::pAUL-A fur mutant | This study |
| Pfl-2 | PFL perR::pAUL-A perR mutant | This study |
| EGDe | Serotype 1/2a | 47 |
| EGDeΔfur | fur deletion mutant of EGDe | 47 |
| Plasmids | ||
| pHK77 | Integratable lacZ fusion vector | 31 |
| pHK77-F | 720-bp upstream region of fur cloned into pHK77 | This study |
| pIntFM | pAUL-A containing 275-bp fur internal PCR fragment | This study |
| pIntPM | pAUL-A containing 275-bp perR internal PCR fragment | This study |
| Primers | ||
| 514F | TATGCGCAAAACTCCATGAA | |
| 514R | CTTTGAGTCCTGCAGCATTTT | |
| FB1F | CGCTTGTTTTACCATACGGAG | |
| FB1R | CCATAAAGTTTTAAGCGATGGC | |
| HK091 | GTTTCCCGCTCGAATTC | |
| HKlacZ | TTCTGGTGCCGGAAACCAG | |
| HKFF | GCTAGCGTTGTGCTTTAATGCGTCCA | |
| HKFR | GGTACCGGATGGCAGGAAATATTTGG | |
| Paf-F | GCAAATGGTACCTGCACCTGACACTGGCCT | |
| Paf-R | TGAATCGGAGCTCCCACTTCGATTCGACGATTT | |
| PaP-F | CCTGAAAGCTTTTAACTCACATACACACCCAACC | |
| pap-R | TGAATGGATCCATATCCGGTCATATGTGCTGC | |
| Svpa1F | AGATGCAATTAGTTATGATCACTGGT | |
| Svpa1R | AGATGCAATTAGTTATGATCACTGGT | |
| qRT-PCR primers | ||
| 16S rRNART-F | CCCTTATGACCTGGGCTACA | |
| 16S rRNART-R | CCTACCGACTTCGGGTGTTA | |
| lmo0485RT-F | CGTATTGCACACACTTGCTG | |
| lmo0485RT-R | CCTGCTTTTAAATCCGCAAT | |
| lmo1956RT-F | TGGCCTTGCAACCGTTTATAG | |
| lmo1956RT-R | TTATCAACCACGCGCAATTC | |
| lmo1960RT-F | CCAACTAGAGACGTTGCCAAA | |
| lmo1960RT-R | ACTTTGATGCGGAGAACGTC | |
| lmo2185RT-F | GATGCAATTAGTTATGATCACTGGT | |
| lmo2185RT-R | CCATCCGAAAGAGTCACAGG |
a
Underlining indicates restriction endonuclease sites.