TABLE 4.

Genes regulated by Fur under iron limitation conditions

Locus tag	Predicted function (gene)	Fold valuea
		Δfur+Fe/WT+Fe	Δfur−Fe/WT−Fe	Δfur−Fe/Δfur+Fe	WT−Fe/WT+Fe
Cell envelope
lmo0366	Similar to putative lipoprotein involved in iron transport	27.61	2.1	—	5.47
Energy metabolism
lmo0485	Nitroreductase family protein	3.83	1.65	1.61	2.17
lmo0943	Nonheme iron-binding ferritin (fri)	2.4	1.89	1.18	2.35
lmo1131	Unknown, similar to ABC transporter, ATP-binding protein	9.93	1.39	—	5.97
Hypothetical proteins
lmo0365	Similar to high-affinity iron transporter	25.03	2.43	—	5.58
lmo1007	Unknown	5.61	—	—	2.48
lmo2132	Unknown	2.04	1.4	1.26	2
lmo2181	Sortase B (srtB)	12.83	1.34	−1.21	4.6
lmo2104	Ferrous iron transport protein (A)	15.17	—	1.22	7
lmo2105	Ferrous iron transport protein (B)	7.76	1.27	—	4.34
Regulatory functions
lmo1956	Ferric uptake regulator	−12.75	−3.25	—	1.44b
Transport and binding proteins
lmo0541	Similar to iron compound binding lipoprotein, ABC Transporter	7.01	—	—	2.62
lmo1957	Ferrichrome ABC transporter (permease)	5.86	1.79	−1.27	2.22
lmo1958	Iron compound ABC transporter, permease protein (fhuB)	4.7	1.44	—	2.01
lmo1960	Iron compound ABC transporter (ATP binding) (fhuC)	2.44	—	—	2.02
lmo2182	Ferrichrome ABC transporter (ATP binding)	13.3	1.34	—	3.97
lmo2183	Ferrichrome ABC Transporter (permease)	15.76	1.41	—	4.48
Unclassified
lmo0361	Twin-arginine-targeting protein translocase (tatC)	22.75	2.85	−1.17	3.61
lmo0362	Twin-arginine-targeting protein translocase (tatA)	19.75	3.21	−1.16	4.29
lmo2180	Unknown, similar to GP157 family of protein	4.09	—	—	4.71
lmo2185	(svpA)	8.91	1.58	—	2.99
lmo2186	Unknown	9.48	—	—	4.24
lmo0367	Putative iron-dependent peroxidase	25.83	1.57	—	4.07
Unknown function
lmo1961	Similar to oxidoreductase	3.58	—	—	2.4

^aNumbers in the fold value column are ratios of expression levels in the fur mutant (Δfur) and in the L. monocytogenes wild type (WT) in the presence of iron (+Fe) or in iron-limiting conditions (−Fe). —, no change or data not available.

^bDownregulated.