FIG. 1.

(A) Results of second reversed-phase chromatography of avicin A. Elution was performed by using a 30-CV linear gradient of 0 to 100% 2-propanol containing 0.1% TFA. Solid line, absorbance at 280 nm; dashed line, isopropanol gradient; bars, bacteriocin units (BU) in eluted active fractions. A sample for mass spectroscopy analysis was obtained from fraction 9. (B) Mass spectrometry analysis of avicin A obtained from the second reversed-phase chromatography. Intens., intensity; a.u., arbitrary units.