TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Source or referenceb |
|---|---|---|
| Strains | ||
| Carnobacterium divergens NCDO 2763T | Sensitive to avicin A | NCDO |
| Carnobacterium divergens NCDO 2306 | Sensitive to avicin A | NCDO |
| Carnobacterium piscicola NCDO 2762T | Sensitive to avicin A | NCDO |
| Carnobacterium piscicola NCDO 2764 | Sensitive to avicin A | NCDO |
| Carnobacterium piscicola UI 49 | Bacteriocin producer | 45 |
| Escherichia coli DH5α | φ80dlacZΔM15 recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+) supE44 relA1 deoR Δ(lacZYA-argF)U169 | Promega |
| Enterococcus avium 208 | Avicin A producer, Norwegian infant isolate | This study |
| Enterococcus avium GM62 | Sensitive to avicin A | This study |
| Enterococcus avium UA62 | Sensitive to avicin A | This study |
| Enterococcus avium UM83 | Sensitive to avicin A | This study |
| Enterococcus avium XA83 | Avicin A producer, Ethiopian infant isolate | This study |
| Enterococcus faecalis 185 | Infant isolate, sensitive to avicin A | This study |
| Enterococcus faecalis LMGT 2708RA | Pediocin-resistant mutant of E. faecalis EF BRIDGE (B) | LMGT |
| Enterococcus faecium P21 | LMGT | |
| Enterococcus maldoratus XM83 | Infant isolate, sensitive to avicin A | This study |
| Enterococcus faecalis EF BRIDGE (B) | Indicator sensitive to avicin A | LMGT |
| Lactobacillus fermentum KLD | LMGT | |
| Lactobacillus plantarum 965 | LMGT | |
| Lactobacillus rhamnosus 205 | Infant isolate, sensitive to avicin A | This study |
| Lactobacillus sakei NCDO 2714 | Indicator sensitive to avicin A | NCDO |
| Lactobacillus sakei 5 | Bacteriocin producer | V. Eijsink |
| Lactococcus lactis IL 1403 | Host for transformation, contains lcnC and lcnD analogues | 49 |
| Lactococcus lactis CM4 | ||
| Leuconostoc gelidum Ta 11a | LMGT | |
| Listeria innocua BL86/26 B | Sensitive to avicin A | LMGT |
| Pediococcus acidilactici | Pediocin producer | LMGT |
| Pediococcus pentosaceus NCDO 559 | NCDO | |
| Plasmids | ||
| pBluescript KS | 3.0-kb cloning vector, αlacZ, Apr | Stratagene |
| pMG36e | 3.7-kb lactococcal expression vector, Emr | 47 |
| pMG36eDV | pMG36e containing divergicin-like ORF, avcB | This study |
| pCR2.1-TOPO | 3.9-kb cloning vector, Apr | Invitrogen |
a
Apr, ampicillin resistant; Emr, erythromycin resistant.
b
NCDO, National Collection of Dairy Organisms (Reading, United Kingdom); LMGT, Laboratory of Microbial Gene Technology, Norwegian University of Life Sciences.