Table 1.

Comparison of in-solution and on-plate digestion for bovine serum albumin, equine myoglobin and bovine insulin standards at 600 ng, 300 ng, 100 ng, 50 ng and 10 ng levels incubated for 5min, 30min, 1h, 3h and 6h. Pept refers to the number of tryptic peptides used for protein identification; Δ refers to the standard deviation in respect to the number of tryptic peptides for the different assays; Score refers to the MASCOT score and % Cov refers to the percentage of coverage from the MASCOT search.

		On-solution digestion
		600ng				300ng				100ng				50ng				10ng
		Pept	Δ	Score	% Cov	Pept	Δ	Score	% Cov	Pept	Δ	Score	% Cov	Pept	Δ	Score	% Cov	Pept	Δ	Score	% Cov
BSA	5 min	7	2	82	13	6	2	75	8	8	2	84	8	6	2	76	8	6	2	4	4
	30min	17	3	184	27	12	3	162	14	11	2	121	14	8	3	104	7	7	2	3	3
	1 h	17	3	196	23	12	3	193	14	17	3	232	18	11	2	149	11	10	2	14	14
	3 h	19	3	400	36	16	3	346	18	15	3	193	22	11	3	146	14	9	3	11	11
	6 h	18	4	335	43	18	3	213	27	14	4	224	23	12	3	112	13	10	2	8	8
Myoglobin	5 min	9	2	150	43	9	2	164	41	6	2	153	41	5	2	63	20	3	2	11	11
	30 min	12	2	194	81	12	2	113	80	6	2	162	43	7	2	121	80	6	2	17	17
	1 h	12	3	278	81	10	2	194	68	7	2	198	69	5	3	155	69	6	2	20	20
	3 h	15	3	226	81	14	3	311	80	14	3	345	80	4	2	114	24	5	3	20	20
	6 h	15	2	500	81	15	3	568	80	13	3	313	80	8	3	167	59	5	2	24	24
Insulin	5 min	4	1	114	100	3	1	94	56	2	1	88	56	2	1	66	56	2	1	56	56
	30 min	3	1	113	100	4	1	69	100	3	2	81	56	2	1	63	56	2	1	56	56
	1 h	4	2	89	100	2	1	91	56	2	1	75	56	2	1	71	56	2	1	56	56
	3 h	4	1	132	100	3	1	104	100	2	1	75	56	3	1	74	56	2	1	56	56
	6 h	3	1	119	100	3	1	109	56	2	1	71	56	2	1	84	56	2	1	56	56
		On-Plate digestion
		600ng				300ng				100ng				50ng				10ng
		Pept	Δ	Score	% Cov	Pept	Δ	Score	% Cov	Pept	Δ	Score	% Cov	Pept	Δ	Score	% Cov	Pept	Δ	Score	% Cov
BSA	5 min	8	2	94	8	7	2	84	8	7	2	74	6	6	2	76	7	6	2	71	7
	30 min	17	3	112	21	16	2	130	17	12	3	121	28	10	3	89	13	8	3	104	8
	1 h	18	4	265	24	18	3	224	29	17	3	221	29	14	2	93	18	11	4	109	11
	3 h	19	4	256	41	19	3	298	41	17	4	362	33	16	3	130	23	12	3	111	8
	6 h	19	3	411	41	19	3	389	41	16	3	246	34	14	2	111	18	9	2	107	14
Myoglobin	5 min	9	2	143	45	10	2	174	33	6	2	254	36	5	2	70	24	4	2	62	12
	30 min	11	3	211	45	13	3	169	43	5	2	193	36	7	2	119	33	6	1	124	16
	1 h	12	2	198	81	10	2	168	81	7	2	162	43	5	2	153	33	6	1	149	18
	3 h	15	5	329	81	15	4	346	81	13	3	384	80	7	2	191	33	5	3	88	24
	6 h	15	4	483	81	15	3	449	81	14	2	511	80	8	3	253	33	5	1	134	24
Insulin	5 min	4	2	99	100	2	1	114	56	2	1	69	56	2	1	88	56	2	1	71	56
	30 min	3	1	105	100	3	1	162	56	3	1	94	56	2	1	71	56	2	1	68	56
	1 h	4	2	154	100	3	1	121	56	3	1	142	56	3	1	82	56	2	1	74	56
	3 h	4	2	123	100	4	1	130	100	3	1	121	56	3	1	91	56	2	1	89	56
	6 h	3	1	142	100	3	1	129	56	3	1	132	56	3	2	84	56	2	1	82	56