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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Mol Microbiol. 2009 Nov 10;74(6):1344–1355. doi: 10.1111/j.1365-2958.2009.06951.x

FIG. 5.

FIG. 5

BosR alters the synthesis of RpoS and virulence determinants regulated by the Rrp2-RpoN-RpoS pathway. Wild type infectious MSK5, parental strain ML23, and JH300 (ML23 bosR::kanR) were grown under anaerobic (A) and microaerobic (M) growth conditions to 5×107 cells/ml. A. Coomassie-stained SDS-PAGE gel of cell lysates. The arrow indicates the presence of a 23-kDa protein in MSK5 and ML23 that is absent in JH300. B. Samples were probed with antibodies specific for the antigen indicated. As in Fig. 4, endoflagellum (EF) is used as a control to normalize for equivalent protein loading.