Table 1.
Strains and Plasmids used in this study
| B. burgdorferi strains used in this study: | ||
| Strain | Genotype | Reference |
| MSK5 | B31 derivative, all plasmids present | (Labandeira-Rey and Skare, 2001a) |
| ML23 | missing lp25 | (Labandeira-Rey and Skare, 2001a) |
| DS102 | ML23, bb0646::gentR | (Hyde et al., 2010) |
| JH300 | ML23, bosR::kanR | This study |
| E. coli strains used in this study: | ||
| Strain | Genotype | Source |
| Mach-1™-T1R | Φ80lacZΔM15 ΔlacX74 hsdR (rk−, mk+) ΔrecA1398 endA1 tonA |
Invitrogen |
| Plasmids used in this study: | ||
| Plasmid | Resistance | Comments/Source/Reference |
| pCR8/GW/TOPO | specR | Gateway PCR cloning/entry vector; Invitrogen |
| pJS167 | kanR | suicide vector containing bosR::kanR construct (Seshu et al., 2004b) |
| pBBE22 | kanR | borrelial shuttle vector pBSV2 containing pncA fragment to restore infectivity in ML23 (Purser et al., 2003) |
| pKFSS1 | specR/strepR | streptomycin resistant borrelial shuttle vector (Frank et al., 2003); confers specR in E. coli |
| pCADDY | specR/strepR | shuttle vector derived from pBBE22 (Purser et al., 2003) replacing the kanamycin resistance with streptomycin resistant determinant; confers specR in E. coli |