FIG. 2.

Relative transcription of EtGFAT in different developmental stages of E. tenella. (A) Analysis of total RNA purified from E. tenella and host tissue using automated electrophoresis. The major rRNA bands, including the 28S (host-specific), 26S (parasite-specific), and 18S (parasite and host), rRNA bands, are marked accordingly. (B) RT-PCR analysis of EtGFAT and EtGam56 transcription. Prior to RT-PCR, each cDNA sample was standardized to comparable levels of EtActin transcription. Merozoites (M), gametocytes (G), unsporulated oocysts (U), sporulated oocysts (S), and uninfected host control (H) are as indicated.