FIG. 8.

Detection of glycosylated proteins in different developmental stages of E. tenella. Immunoblots of whole-cell protein lysates from E. tenella and host tissue were probed with WGA or JAC for the detection of proteins glycosylated with N-acetylglucosamine or N-acetylgalactosamine, respectively. A unique gametocyte-specific band (open white arrow) and unsporulated oocyst-upregulated band (asterisk), both JAC positive, are marked accordingly. Size markers (in kilodaltons) are displayed to the left of each lectin blot. Merozoites (M), gametocytes (G), unsporulated oocysts (U), sporulated oocysts (S), and uninfected host control (H) are as indicated.