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. 2009 Nov 13;9(1):164–172. doi: 10.1128/EC.00194-09

FIG. 4.

FIG. 4.

RT-PCR analysis of MATORF1 and MATORF2 expression in Mycosphaerella fijiensis. The isolates used were the MAT1-1 isolate CIRAD86 and the M. fijiensis MAT1-2 isolate CIRAD251. PCR with primer combinations ORF1F/ORF1R (lanes 1 to 4) and ORF2F/ORF2R (lanes 5 to 8) was performed on cDNA derived from CIRAD251 (lanes 1 and 5), cDNA derived from CIRAD86 (lanes 2 and 6), genomic DNA from CIRAD251 (lanes 3 and 7), and genomic DNA from CIRAD86 (lanes 4 and 8). The lane labeled M contains a DNA marker. The numbers to left and right show nucleotide sizes of amplified fragments.