FIG. 6.

Group A compounds cause accumulation of Golgi or endosome-like membranes. An apl2Δ strain (EHY807 [A and B]) or wild-type (EHY47 [E]) were grown in the presence of 2.5 μM compound KU#7 for 30 min. (C) Similar results were obtained for apl2Δ cells grown in 10 μM KU#3 (a less-potent group A compound) for 60 min. DMSO controls without compound are shown in panels D (apl2Δ) and F (wild type). Cells were prepared for thin-section electron microscopy as described previously (18). Scale bars: 200 nm (A), 500 nm (B to F).