FIG. 2.

Direct comparison of Dia2 and Cdc4 indicate that Cdc4 is the F-box that recognizes phosphorylated Tec1. (A) Shown is an immunoblot of a pheromone time course performed at 37°C with wild-type and cdc4-1 cells transformed with myc-Tec1 expressed from its native promoter on a CEN plasmid. Cells were shifted to the nonpermissive temperature for 1 h prior to treatment with pheromone. (B) Shown is an immunoblot of a pheromone time course with wild-type and dia2Δ strains.