TABLE 1.
Summary of analysis of Tec1 mutantsa
| Tec1 allele | Protein stability in pheromone-treated cellsb | Mean β-galactosidase activity ± SD (FRE-lacZ) |
|---|---|---|
| Wild type | - | 1 |
| S263A | - | 1.19 ± 0.25 |
| G264A | - | 0.96 ± 0.2 |
| L265A | - | 0.84 ± 0.02 |
| S266A | - | 1.01 ± 0.14 |
| V267A | - | 0.97 ± 0.03 |
| H268A | - | 1.02 ± 0.09 |
| S269A | - | 1.18 ± 0.40 |
| K270R | - | 0.97 ± 0.26 |
| L271A | -/+ | 1.01 ± 0.12 |
| L272A | -/+ | 1.10 ± 0.04 |
| T273 M | ++ | 3.27 ± 0.24 |
| P274S | ++ | 2.76 ± 0.41 |
| T276A | ++ | 3.55 ± 0.52 |
| A277V | + | 1.74 ± 0.16 |
| S278A | + | 1.25 ± 0.11 |
| N279A | - | 0.82 ± 0.18 |
| E280A | + | 1.31 ± 0.17 |
| K281R | - | 0.95 ± 0.12 |
| I283A | + | 1.05 ± 0.13 |
| E284A | - | 0.85 ± 0.08 |
| N285A | - | 0.94 ± 0.05 |
| T289A | - | 1.27 ± 0.10 |
| T297A | - | 1.27 ± 0.28 |
a
Shown is a summary of immunoblotting time course experiments of cells treated with mating pheromone (second column), as well as an examination of basal FRE-lacZ expression (third column). The wild type was set to a value of 1.
b
-, Wild-type destruction of myc-Tec1 in pheromone-treated cells over a 1-h time course; ++, no destruction; +, partial destruction; +/-, slight destruction.