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Hyperelongation of glutamyl side chains in the cell body causes accumulation of α-tubulin K40 acetylation. (A) Wild-type and GFP-Ttll6Ap cells (indicated by the asterisk) were grown for 4 h in the presence of 2.5 μg of CdCl2/ml and labeled side-by-side with antiacetylated tubulin antibodies (6-11 B-1). (A) Projection image of z-section from the top half of the cell; (A′) section showing the middle part of the cell. Bar, 10 μm.
