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. 2009 Aug 21;9(1):184–193. doi: 10.1128/EC.00176-09

FIG. 5.

FIG. 5.

Hyperelongation of glutamyl side chains delays cilium regeneration. (A to F) Confocal immunofluorescence images of wild-type (A and B) and GFP-Ttll6Ap (C and D)-, GFP-Ttll6Ap-M241-V929-E662G (E)-, and GFP-Ttll6Ap-M241-V929 (F)-overexpressing cells that regenerated cilia for 1 (A and C), 2 (E and F), or 4 h (B and D) after deciliation. Cells were costained with anti-α-tubulin 12G10 MAb and the anti-polyglutamylation antibody poly(E). Note the shorter size of cilia of GFP-Ttll6Ap cells. Insets show cilia at higher magnifications from the areas indicated by a bracket. (G) A graph shows the average lengths of cilia as a function of time of cilium regeneration. Bar, 10 μm.