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. 2009 Aug 21;9(1):184–193. doi: 10.1128/EC.00176-09

FIG. 7.

FIG. 7.

Paclitaxel partly rescues the destabilizing effect of hyperglutamylation on elongation of assembling cilia in vegetatively growing Tetrahymena. Confocal immunofluorescence images of wild-type (A and B) and GFP-Ttll6Ap-overexpressing (D and E) cells grown without (A and D) or with (B and E) 40 μM paclitaxel. Cells were costained with anti-α-tubulin antibodies and anti-poly(E) antibodies. The arrows in panel E point to hyperglutamylated distal segments. Bar, 10 μm. (C and F) A graph documents the distribution of cilium length in wild-type (C) and GFP-Ttll6Ap-overexpressing (F) cells. Black diamonds indicate the cilium lengths in cells grown without drug, and open triangles represent the cilium lengths in cells grown in the presence of paclitaxel.