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C. neoformans APT1 restores the growth of an S. cerevisiae drs2 mutant. The C. neoformans APT1 (CnAPT1) gene was expressed in the drs2 mutant of S. cerevisiae (drs2 + CnAPT1). The drs2 mutant is able grow at 30°C but not at 18°C. Tenfold serial dilutions of yeast cells were plated on YPD or YPD with calcofluor white (CFW) and cultured at 18°C or 30°C. The plates were incubated for 3 days.
