FIG. 5.

Apt1 is required for virulence in a mouse inhalation model. (A) Three female A/Jcr mice were inoculated intranasally with each of the strains indicated, and the survival of the mice was monitored twice per day. The apt1 mutants showed a difference in virulence from the WT strain, H99 (P = 0.0246), by the log rank test. (B) Ten female BALB/c mice were challenged by intranasal inoculation with 10⁶ cells of either the WT strain, H99, or two independent apt1 mutant strains. The survival of the mice was monitored twice per day. The apt1 mutants showed a difference in virulence from the WT strain (P < 0.001) by the log rank test.